Haynes Lab:Notebook/Engineering PC-TFs/2012/09/28: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2012/09/28 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
(21 intermediate revisions by the same user not shown)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Summary==
==Summary==
*  
* Thaw and dilute primers (x10 dilution)
*90 μL water
*10 μL primer stock
 
(10 μm in each tube for PCR reaction)
 
*Assemblies
 
1. hPCD - Pflex - BL01<br>
2. hPCD - BL01
 
 
{|border="1" cellpadding="5" cellspacing="0" align="left"
|-
! scope="col" style="background:#efefef;" | #
! scope="col" style="background:#efefef;" | Primer
 
|-
|1
|BBP-hPCD fwd
|-
|2
|hPCD - Plflex rev
|-
|3
|hPCD-Plflex fwd
|-
|4
|Plfex-BL01 rev
|-
|5
|Plflex-BL01 fwd
|-
|6
|BL01-BBS rev
|-
|7
|hPCD-BL01 rev
|-
|8
|hPCD-BL01 fwd
|-
|}
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
{|border="1" cellpadding="5" cellspacing="0" align=";left"
|-
! scope="col" style="background:#efefef;" | #
! scope="col" style="background:#efefef;" | Template
! scope="col" style="background:#efefef;" | Primers
|-
|1
|hPCD
|1, 2
|-
|2
|Plflex
|3, 4
|-
|3
|BL01
|5,6
|-
|4
|hPCD
|1, 7
|-
|5
|BL01
|8,6
|-
|}
 
{|border="1" cellpadding="5" cellspacing="0" align="left"
|-
! scope="col" style="background:#efefef;" |
! scope="col" style="background:#efefef;" | μL
|-
|Template
|.5 μL
|-
|Primer 1 (10 μm)
|1.0 μL
|-
|Primer 2 (10 μm)
|1.0 μL
|-
|2*GoTaq
|12. 5
|-
|DH2O
|10.0 μL
|-
|}




Revision as of 13:59, 29 September 2012

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Thaw and dilute primers (x10 dilution)
  • 90 μL water
  • 10 μL primer stock

(10 μm in each tube for PCR reaction)

  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01


# Primer
1 BBP-hPCD fwd
2 hPCD - Plflex rev
3 hPCD-Plflex fwd
4 Plfex-BL01 rev
5 Plflex-BL01 fwd
6 BL01-BBS rev
7 hPCD-BL01 rev
8 hPCD-BL01 fwd
















# Template Primers
1 hPCD 1, 2
2 Plflex 3, 4
3 BL01 5,6
4 hPCD 1, 7
5 BL01 8,6
μL
Template .5 μL
Primer 1 (10 μm) 1.0 μL
Primer 2 (10 μm) 1.0 μL
2*GoTaq 12. 5
DH2O 10.0 μL



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/09/28&oldid=630838"