Haynes Lab:Notebook/Engineering PC-TFs/2012/09/28: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 80: Line 80:
|-
|-
|}
|}
<br>
<br><br><br><br><br>




Revision as of 13:43, 29 September 2012

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Thaw and dilute primers (x10 dilution)
  • 90 μL water
  • 10 μL primer stock

(10 μm in each tube for PCR reaction)

  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01


# Primer
1 BBP-hPCD fwd
2 hPCD - Plflex rev
3 hPCD-Plflex fwd
4 Plfex-BL01 rev
5 Plflex-BL01 fwd
6 BL01-BBS rev
7 hPCD-BL01 rev
8 hPCD-BL01 fwd



# Template Primers
1 hPCD 1, 2
2 Plflex 3, 4
3 BL01 5,6
4 hPCD 1, 7
5 BL01 8,6








Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/09/28&oldid=630823"