Haynes Lab:Notebook/Engineering PC-TFs/2012/10/19: Difference between revisions
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(Autocreate 2012/10/19 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs) |
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==Summary== | ==Summary== | ||
* | *Retrieve gel slices from fridge. | ||
*Add 3 volumes of ADB Buffer to each volume of gel. (Each gel slice is assumed to be 200 mg) | |||
*Therefore, add 600 μL of ADB Buffer. | |||
*Incubate at 55°C for 10 minutes. | |||
*Load melted agarose solution into Spin Column in a collection tube. | |||
*Centrifuge at max speed for 30 seconds. | |||
*Discard flow-through. | |||
*Add 200 μL of DNA wash buffer to the spin columns and centrifuge for 30 seconds. | |||
*Place spin column in a 1.5 mL tube. Add 10μL of DNA Elution Buffer to elute the DNA. | |||
*Place tubes into freezer box. | |||
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Revision as of 15:57, 23 October 2012
 Engineering PC-TFs
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