Haynes Lab:Notebook/Engineering PC-TFs/2012/10/26: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(5 intermediate revisions by the same user not shown) | |||
Line 63: | Line 63: | ||
<br> | <br> | ||
<br> | <br> | ||
{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
Line 101: | Line 100: | ||
|2.0 μL | |2.0 μL | ||
|- | |- | ||
|Total | |<i>Total</i> | ||
|5.0 μL | |5.0 μL | ||
|5.0 μL | |5.0 μL | ||
|} | |} | ||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
<br> | |||
*Thaw DHSα Turbo cells (50 μL per transformation) | |||
*Add 20 μL of Gibson reaction to 50μL DHSα Turbo. | |||
**Assembly 1 | |||
**Assembly 2 | |||
**Negative Control (DH2O) | |||
*Incubate on ice for five minutes. | |||
*Plate 70 μL transformation on warm Amp plates. | |||
Revision as of 13:39, 30 October 2012
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||
Summary
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.
|