Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06: Difference between revisions
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{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
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! scope="col" style="background: | ! scope="col" style="background:#efefef;" | | ||
! scope="col" style="background:#efefef;" | 1 | ! scope="col" style="background:#efefef;" | 1 | ||
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---- | ---- | ||
PCR Check | '''PCR Check''' | ||
# Product 1: outer-most primers for whole assembly | # Product 1: outer-most primers for whole assembly | ||
# Product 1: Gibson primer pair for hPCD | # Product 1: Gibson primer pair for hPCD | ||
# Product 2: outer-most primers | # Product 2: outer-most primers | ||
# Product 2: Gibson primer pair for hPCD | # Product 2: Gibson primer pair for hPCD | ||
<br> | |||
{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
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| <i>Reagent</i> || | | <i>Reagent</i> || 1 || 2 || 3 || 4 | ||
|- | |- | ||
| | | Gibson product || 0.5 μL || 0.5 || 0.5 || 0.5 | ||
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|- | |- | ||
| | | primer 1 || 1.0 || 1.0 || 1.0 || 1.0 | ||
|1.0 | |||
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|- | |- | ||
| | | primer 2 || 1.0 || 1.0 || 1.0 || 1.0 | ||
|1.0 | |||
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|- | |- | ||
| | | 2x GoTaq PCR mix || 14.5 || 14.5 || 14.5 || 14.5 | ||
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|- | |- | ||
| | | dH<sub>2</sub>O || 12.0 || 12.0 || 12.0 || 12.0 | ||
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|} | |} | ||
<br> | <br> |
Revision as of 16:52, 6 November 2012
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Summary
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.
PCR Check
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