Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06: Difference between revisions
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Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. | Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. | ||
There is no addition of vector this time. | There is no addition of vector this time. | ||
{|border="1" cellpadding="5" cellspacing="0 | {|border="1" cellpadding="5" cellspacing="0" | ||
|- | |- | ||
! | ! style="background:#efefef;" | | ||
! | ! style="background:#efefef;" | 1 | ||
! | ! style="background:#efefef;" | 2 | ||
|- | |- | ||
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|} | |} | ||
{|border="1" cellpadding="5" cellspacing="0 | {|border="1" cellpadding="5" cellspacing="0" | ||
|- | |- | ||
! scope="col" style="background:#efefef;" | <i>DNA fragments</i> | ! scope="col" style="background:#efefef;" | <i>DNA fragments</i> | ||
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|5.0 μL | |5.0 μL | ||
|} | |} | ||
<br> | <br><br><br><br> | ||
---- | ---- | ||
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<br> | <br> | ||
{|border="1" cellpadding="5" cellspacing="0 | {|border="1" cellpadding="5" cellspacing="0" | ||
|- | |- | ||
| <i>Reagent</i> || 1 || 2 || 3 || 4 | | <i>Reagent</i> || 1 || 2 || 3 || 4 | ||
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<br> | <br> | ||
<br> | <br> | ||
Program: | |||
* 95°C, 3 min. | |||
* [95°C, 30 sec; 57°C, 30 sec; 72°C, 30 sec] 30 cycles | |||
* 72°C, 3 min. | |||
* 4°C ∞ | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 16:55, 6 November 2012
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Summary
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.
PCR Check
Program:
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