Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06: Difference between revisions
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(Autocreate 2012/11/06 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs) |
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==Summary== | ==Summary== | ||
* | * <u>Gibson Assembly </u> | ||
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. | |||
There is no addition of vector this time. | |||
{|border="1" cellpadding="5" cellspacing="0" align="left" | |||
|- | |||
! scope="col" style="background:#efefef;" | | |||
! scope="col" style="background:#efefef;" | 1 | |||
! scope="col" style="background:#efefef;" | 2 | |||
|- | |||
|Gibson Rxn mix | |||
|15 μL | |||
|15 μL | |||
|- | |||
|DNA fragments | |||
|5 μL | |||
|5 μL | |||
|} | |||
<br> | |||
<br> | |||
<br> | |||
Revision as of 16:23, 6 November 2012
 Engineering PC-TFs
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Summary
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.
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