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 Engineering PC-TFs
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Summary
- Performed another PCR run because lid was not closed entirely yesterday. Next time in lab(Monday), confirmation gel will be run to show that PCR rxns worked.
1. hPCD - Pflex - BL01
2. hPCD - BL01
| #
|
Template
|
Primers
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| 1
|
hPCD
|
9, 2
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| 2
|
Plflex
|
3, 4
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| 3
|
BL01
|
5,10
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| 4
|
hPCD
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9, 7
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| 5
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BL01
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8,10
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μL
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| Template
|
.5 μL
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| Primer 1 (10 μm)
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1.0 μL
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| Primer 2 (10 μm)
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1.0 μL
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| 2*GoTaq
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12. 5
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| DH2O
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10.0 μL
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PCR was set with preset settings:
- Ran Zymoclean DNA recovery kit.
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Engineering PC-TFs
