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Haynes Lab:Notebook/Engineering PC-TFs/2012/12/10 - Revision history
2013-06-19T17:06:54Z
Revision history for this page on the wiki
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http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663673&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:37:18Z
<p><span class="autocomment">Summary</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 08:37, 14 December 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012plateread.jpg|150px||left|]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012plateread.jpg|150px||left|]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br><br><br><br><br><br><br><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins><br><br><br><br><br><br><br><br><br><br></div></td></tr>
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Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663672&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:37:02Z
<p><span class="autocomment">Summary</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 08:37, 14 December 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Spin Column for each PCR rxn placed in new 1.5 mL tube.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Spin Column for each PCR rxn placed in new 1.5 mL tube.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*30μL of water was direct added to the column to elute DNA.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*30μL of water was direct added to the column to elute DNA.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012plateread.jpg|150px||left|]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012plateread.jpg|150px||left|]]</div></td></tr>
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Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663671&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:36:43Z
<p><span class="autocomment">Summary</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 08:36, 14 December 2012</td>
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Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663670&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:36:24Z
<p><span class="autocomment">Summary</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 08:36, 14 December 2012</td>
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Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663668&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:35:43Z
<p><span class="autocomment">Summary</span></p>
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:35, 14 December 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*30μL of water was direct added to the column to elute DNA.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*30μL of water was direct added to the column to elute DNA.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:December_12_2012plateread.jpg|400px||left|]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br><br><br><br><br><br><br><br><br><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
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Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663667&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:28:50Z
<p><span class="autocomment">Summary</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:28, 14 December 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Zymo DNA Clean & Concentrator Kit</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Zymo DNA Clean & Concentrator Kit</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Follow these steps for Zymo DNA Clean % Concentrator Kit</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Add 30 μL of DNA Binding Buffer to each 15 μL PCR Reaction (#1,2,3,4,5)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Add 30 μL of DNA Binding Buffer to each 15 μL PCR Reaction (#1,2,3,4,5)</div></td></tr>
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</table>
Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663666&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:28:14Z
<p><span class="autocomment">Summary</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:28, 14 December 2012</td>
</tr>
<tr><td colspan="2" class="diff-lineno">Line 25:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012.jpg|400px||left|]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012.jpg|400px||left|]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><br><br><br><br><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br><br><br><br><br><br><br><br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">----</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Zymo DNA Clean & Concentrator Kit</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Add 30 μL of DNA Binding Buffer to each 15 μL PCR Reaction (#1,2,3,4,5)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Pipette up and down mixture several times to make sure it's thoroughly mixed.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Centrifuged at top speed for 30 seconds. Flow through was discarded.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Added 200 μL of Wash Buffer the column and centrifuged for 30 seconds. Repeated step.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*Spin Column for each PCR rxn placed in new 1.5 mL tube.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*30μL of water was direct added to the column to elute DNA.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
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</table>
Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663665&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:23:25Z
<p><span class="autocomment">Summary</span></p>
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:23, 14 December 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012.jpg|400px||left|]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012.jpg|400px||left|]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><br><br><br><br><br></ins><br><br><br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
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</table>
Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663664&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:22:54Z
<p><span class="autocomment">Summary</span></p>
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:22, 14 December 2012</td>
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<tr><td colspan="2" class="diff-lineno">Line 24:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012.jpg|400px||left|]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:December_12_2012.jpg|400px||left|]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><br><br></ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
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</table>
Brady S. Laughlin
http://www.openwetware.org/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/12/10&diff=663663&oldid=prev
Brady S. Laughlin: /* Summary */
2012-12-14T08:22:36Z
<p><span class="autocomment">Summary</span></p>
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:22, 14 December 2012</td>
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Brady S. Laughlin