The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry <html> </html>Next entry <html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
01/25/13
Type IIS assembly - day 1
PCR
Resuspend new primers to a final concentration of 100 μM
Make 10 μM dilutions in separate tubes
All of the reactions are as follows:
pSB1A3, gg0001, gg0002
hPCD, gg0003, gg0004
BL02, gg0005, gg0007
BL03, gg0005, gg0008
BL04, gg0005, gg0009
fshPCD, gg0010, gg0012
Reagent
Volume
Expected: 1. pSB1A3 = ~2000 2. hPCD = 186 3. BL02 = 1431 4. BL03 = 1731 5. BL04 = 1686 6.fshPCD = 186
10 μL/lane; 1% agarose; Ladder
DNA(plasmid)
0.2 μL
primer 1
1.0
primer 2
1.0
2x GoTaq
25.0
dH2 O
22.8
50.0 μL
95°C, 3 min.
[95°C, 30 sec.; 57°C, 30 sec.; 72°C, 1 min.] x35
72°C, 3 min.
4°C, ∞
Purification (Completed on 1-25-2013)
Clean with Zymo DNA c&c; elute w/ 20 μL dH2 O
Measure [DNA] (1-29-2013)
Sample
OD 260
260/280
ng/μL
1. pSB1A3
.084
1.801
83.954
2. hPCD
.017
1.734
17.344
3. BL02
.058
1.764
58.413
4. BL03
.082
1.831
81.877
5. BL04
.05
1.888
49.561
6. fshPCD
.021
1.622
21.378