Haynes Lab:Notebook/Engineering PC-TFs/2013/01/29: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 14: | Line 14: | ||
** Formula: x = '''length in bp''' ÷ '''measured ng/μL''' * 0.26 | ** Formula: x = '''length in bp''' ÷ '''measured ng/μL''' * 0.26 | ||
|- | |- | ||
| '''Perform BsmBI/ T4 ligase mediated assembly''' | | '''Perform BsmBI/ T4 ligase mediated assembly''' | ||
* BsmBI cuts the DNA fragments and creates complementary overhangs. | * BsmBI cuts the DNA fragments and creates complementary overhangs. |
Revision as of 00:02, 1 February 2013
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||
Summary | |||||||||||||||
Digestion/ ligation reaction |
Dilute the purified PCR product to 20 fmol/μL
| ||||||||||||||
Perform BsmBI/ T4 ligase mediated assembly
|
|}