Haynes Lab:Notebook/Engineering PC-TFs/2013/01/29: Difference between revisions
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==Summary== | ==Summary== | ||
<br><font size=3>'''Digestion/ ligation reaction'''</font> | |||
* <br>'''Dilute the purified PCR product to 20 fmol/μL''' | * <br>'''Dilute the purified PCR product to 20 fmol/μL''' | ||
* Measure ng/μL of the purified sample. | * Measure ng/μL of the purified sample. | ||
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* T4 ligase seals gaps in the phosphodiester DNA backbone. | * T4 ligase seals gaps in the phosphodiester DNA backbone. | ||
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| bgcolor="grey" | Reagent | | bgcolor="grey" | Reagent | ||
| bgcolor="grey" | Vol. | | bgcolor="grey" | Vol. |
Revision as of 00:04, 1 February 2013
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