Haynes Lab:Notebook/Engineering PC-TFs/2013/01/29: Difference between revisions
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| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==1/29/13== | ||
Type IIS Assembly Day 2 | |||
---- | |||
<br><font size=3>'''Digestion/ ligation reaction'''</font> | <br><font size=3>'''Digestion/ ligation reaction'''</font> | ||
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|- | |- | ||
| || 10.0 μL | | || 10.0 μL | ||
|} | |||
{| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | |||
|- valign="top" | |||
| bgcolor="grey" | Reagent | |||
| bgcolor="grey" | 1 | |||
| bgcolor="grey" | 2 | |||
| bgcolor="grey" | 3 | |||
|- | |||
| gg2 pSB1A3 || 1.0 || 1.0 || 1.0 | |||
|- | |||
| gg3 hPCD || 1.0 || 1.0 || 1.0 | |||
|- | |||
| BL02 || 1.0 || --- || --- | |||
|- | |||
| BL03 || --- || 1.0 || --- | |||
|- | |||
| BL04 || --- || --- || 1.0 | |||
|- | |||
| 10x ligase buffer || 1.0 || 1.0 || 1.0 | |||
|- | |||
| NEB T4 lgase || 0.25 || 0.25 || 1.0 | |||
|- | |||
| BsmBI || 0.5 || 0.5 || 0.5 | |||
|- | |||
| dH<sub>2</sub>O || 5.25 || 5.25 || 5.25 | |||
|- | |||
| Total|| 10.0 || 10.0 | |||
|} | |} | ||
Revision as of 20:58, 1 February 2013
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1/29/13Type IIS Assembly Day 2
Purified PCR Product to 20fmol/μL
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