Haynes Lab:Notebook/Engineering PC-TFs/2013/01/29: Difference between revisions
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|style="background-color: # | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: # | |style="background-color: #800000" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==1/29/13== | ||
Type IIS Assembly Day 2 | |||
---- | |||
<br><font size=3>'''Digestion/ ligation reaction'''</font> | <br><font size=3>'''Digestion/ ligation reaction'''</font> | ||
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** Formula: x = '''length in bp''' ÷ '''measured ng/μL''' * 0.26 | ** Formula: x = '''length in bp''' ÷ '''measured ng/μL''' * 0.26 | ||
<font size=3>'''Purified PCR Product to 20fmol/μL'''</font> | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
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* 4°C, ∞ | * 4°C, ∞ | ||
|- | |- | ||
| 20 fmol of each DNA part || up to 8.0 | | 20 fmol (1 μL) of each DNA part || up to 8.0 | ||
|- | |- | ||
| 10x T4 ligase buffer (Promega) || 1.0 | | 10x T4 ligase buffer (Promega) || 1.0 | ||
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| || 10.0 μL | | || 10.0 μL | ||
|} | |} | ||
{| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | |||
|- valign="top" | |||
| bgcolor="grey" | Reagent | |||
| bgcolor="grey" | 1 | |||
| bgcolor="grey" | 2 | |||
| bgcolor="grey" | 3 | |||
|- | |||
| gg2 pSB1A3 || 1.0 || 1.0 || 1.0 | |||
|- | |||
| gg3 hPCD || 1.0 || 1.0 || 1.0 | |||
|- | |||
| BL02 || 1.0 || --- || --- | |||
|- | |||
| BL03 || --- || 1.0 || --- | |||
|- | |||
| BL04 || --- || --- || 1.0 | |||
|- | |||
| 10x ligase buffer || 1.0 || 1.0 || 1.0 | |||
|- | |||
| NEB T4 lgase || 0.25 || 0.25 || 1.0 | |||
|- | |||
| BsmBI || 0.5 || 0.5 || 0.5 | |||
|- | |||
| dH<sub>2</sub>O || 5.25 || 5.25 || 5.25 | |||
|- | |||
| Total|| 10.0 || 10.0 | |||
|} | |||
Revision as of 18:30, 6 September 2013
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1/29/13Type IIS Assembly Day 2
Purified PCR Product to 20fmol/μL
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