Haynes Lab:Notebook/Engineering PC-TFs/2013/01/29: Difference between revisions
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Line 62: | Line 62: | ||
|- | |- | ||
| || 10.0 μL | | || 10.0 μL | ||
|} | |||
{| {{table}} border="1" cellspacing="3" <!-- Golden Gate Rxn. table --> | |||
|- valign="top" | |||
| Reagent || 1 || 2 || 3 | |||
|- | |||
| gg2 pSB1A3 || 1.0 || 1.0 || 1.0 | |||
|- | |||
| gg3 hPCD || 1.0 || 1.0 || 1.0 | |||
|- | |||
| BL02 || 1.0 || --- || --- | |||
|- | |||
| BL03 || --- || 1.0 || --- | |||
|- | |||
| BL04 || --- || --- || 1.0 | |||
|- | |||
| 10x ligase buffer || 1.0 || 1.0 || 1.0 | |||
|- | |||
| NEB T4 lgase || 0.25 || 0.25 || 1.0 | |||
|- | |||
| BsmBI || 0.5 || 0.5 || 0.5 | |||
|- | |||
| dH<sub>2</sub>O || 5.25 || 5.25 || 5.25 | |||
|- | |||
| || 10.0 || 10.0 | |||
|} | |} | ||
Revision as of 20:55, 1 February 2013
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1/29/13Type IIS Assembly Day 2
Purified PCR Product to 20fmol/μL
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