Haynes Lab:Notebook/Engineering PC-TFs/2013/03/05: Difference between revisions
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[[Image:gel3_5_2013.jpg| | [[Image:gel3_5_2013.jpg|200px|Stock digest 3/5/2013]] | ||
[[Image:gel3_5_2013_cutout.jpg| | [[Image:gel3_5_2013_cutout.jpg|200px|Stock digest 3/5/2013]] | ||
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*<font size=4>''Transformation of hPCD, fshPCD, flyPCD'' | *<font size=4>''Transformation of hPCD, fshPCD, flyPCD'' |
Revision as of 20:12, 5 March 2013
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
A) Get biobricks and enzymes from freezer. SpeI, PstI, XbaI The following list of parts are:
B)Follow digest procedure: DNA 20.0 µL Enzyme 1 1.0 µL Enzyme 2 1.0 µL 10x buffer 3.0 µL dH2O 5.0 uL = 30.0 uL
Biobrick Restriction Enzymes
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