Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18: Difference between revisions
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|style="background-color: # | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: # | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==3/18/22== | ||
Cutting inserts to be placed in mammalian vector | Cutting inserts to be placed in mammalian vector | ||
''' A) Get biobricks and enzymes from freezer.''' | ''' A) Get biobricks and enzymes from freezer.''' | ||
PstI, XbaI | |||
The following list of parts are: | The following list of parts are: | ||
* BL01 | * 1. BL01 | ||
* BL05 | * 2. BL05 | ||
* BL09 | * 3. BL09 | ||
* BL12 | * 4. BL12 | ||
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<font size=3>''B)Follow digest procedure:'' | <font size=3>''B)Follow digest procedure:'' | ||
*DNA 20.0 µL | |||
*Enzyme 1 1.0 µL | |||
*Enzyme 2 1.0 µL | |||
*10x buffer 3.0 µL | |||
*dH2O 5.0 uL | |||
* = 30.0 uL </font> | |||
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Latest revision as of 22:34, 26 September 2017
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3/18/22Cutting inserts to be placed in mammalian vector A) Get biobricks and enzymes from freezer. PstI, XbaI The following list of parts are:
Digest Order: Biobrick Restriction Enzymes
Follow steps for gel electrophoresis.
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