Haynes Lab:Notebook/Engineering PC-TFs/2013/03/25: Difference between revisions
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Line 15: | Line 15: | ||
The following list of parts are: | The following list of parts are: | ||
* Kozak | * Kozak | ||
Follow steps for gel electrophoresis. | Follow steps for gel electrophoresis. | ||
Line 45: | Line 33: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:gel3_25_2013.jpg|200px|Stock digest 3/25/2013]] [[Image:gel3_25_2013_cutout.jpg|200px|Stock digest 3/18/2013]] <br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA (plasmid) || | | DNA (plasmid) || 20.0 μL | ||
|- | |- | ||
| | | SpeI|| 1.0 μL | ||
|- | |- | ||
| PstI|| 1.0 μL | | PstI|| 1.0 μL | ||
|- | |- | ||
| Green 10x FastDigest buffer || | | Green 10x FastDigest buffer || 3.0 μL | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 5 μL | ||
|- | |- | ||
| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. |
Revision as of 13:36, 26 March 2013
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Summary
A) Get biobricks and enzymes from freezer. PstI, SpeI The following list of parts are:
Follow steps for gel electrophoresis.
|