Haynes Lab:Notebook/Engineering PC-TFs/2013/03/27: Difference between revisions

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(Autocreate 2013/03/27 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
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==Summary==
==3/27/2013==
*  
 
{| {{table}}
| align="center" style="background:#0095B6;"|
| align="center" style="background:#0095B6;"|'''1'''
| align="center" style="background:#0095B6;"|'''2'''
| align="center" style="background:#0095B6;"|'''3'''
| align="center" style="background:#0095B6;"|'''4'''
| align="center" style="background:#0095B6;"|'''5'''
|-
| DNA Insert||1.4||1.8||2.0||2.5||--
|-
| DNA Vector (Kozak)||0.6||0.6||0.6||0.6||0.6
|-
| T4 Ligase||1||1||1||1||1
|-
| Lign Buffer (2x)||5||5||5||5||5
|-
| dH2O||2.0||1.6||1.4||.9||3.7
|-
| ||10 µL||10 µL||10 µL||10 µL||10 µL
|-
|
|}
 
* In a .5 mL tube, pipette the ligation buffer first.
* Then water, DNA insert, vector, and T4 ligase.
* Once completed, allow them to incubate for 10 minutes.
* Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes.
* Then follow transformation process:




Revision as of 09:05, 2 April 2013

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3/27/2013

1 2 3 4 5
DNA Insert 1.4 1.8 2.0 2.5 --
DNA Vector (Kozak) 0.6 0.6 0.6 0.6 0.6
T4 Ligase 1 1 1 1 1
Lign Buffer (2x) 5 5 5 5 5
dH2O 2.0 1.6 1.4 .9 3.7
10 µL 10 µL 10 µL 10 µL 10 µL
  • In a .5 mL tube, pipette the ligation buffer first.
  • Then water, DNA insert, vector, and T4 ligase.
  • Once completed, allow them to incubate for 10 minutes.
  • Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes.
  • Then follow transformation process:



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