Haynes Lab:Notebook/Engineering PC-TFs/2013/03/27: Difference between revisions
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|10.84 | |10.84 | ||
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|}<br><br><br><br><br><br><br> | |}<br><br><br><br><br><br><br> | ||
{| {{table}} | |||
|align="center" style="background:#f0f0f1;"|'''µL of insert = (length insert/lengthvector *2*25 ng vector/concentration insert)''' | |||
|} | |||
{| {{table}} | {| {{table}} | ||
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*Add 30 μL of DH5α Turbo cells to DNA + dH2O | *Add 30 μL of DH5α Turbo cells to DNA + dH2O | ||
*Include #5 tube, water only, no DNA (negative control) | *Include #5 tube, water only, no DNA (negative control) | ||
*Incubate cells + DNA on ice for | *Incubate cells + DNA on ice for 10 min. | ||
*Label pre-warmed plates | *Label pre-warmed plates | ||
*Transfer cells + DNA onto agar | *Transfer cells + DNA onto agar |
Revision as of 10:55, 2 April 2013
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3/27/2013
Transformation Process
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