Haynes Lab:Notebook/Engineering PC-TFs/2013/03/27: Difference between revisions
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|style="background-color: # | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: # | |style="background-color: #800000" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==3/27/2013== | ==3/27/2013== | ||
*1. Kozak,V0120 + BL01 | *1. Kozak,V0120 + BL01 | ||
*2. Kozak,V0120 + BL05 | *2. Kozak,V0120 + BL05 | ||
*3. Kozak,V0120 + BL09 | *3. Kozak,V0120 + BL09 | ||
*4. Kozak,V0120 + BL12 | *4. Kozak,V0120 + BL12 | ||
{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
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|10.84 | |10.84 | ||
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|align="center" style="background:#f0f0f1;"|'''µL of insert = (length insert/lengthvector *2*25 ng vector/concentration insert)''' | |||
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*Add 30 μL of DH5α Turbo cells to DNA + dH2O | *Add 30 μL of DH5α Turbo cells to DNA + dH2O | ||
*Include #5 tube, water only, no DNA (negative control) | *Include #5 tube, water only, no DNA (negative control) | ||
*Incubate cells + DNA on ice for | *Incubate cells + DNA on ice for 10 min. | ||
*Label pre-warmed plates | *Label pre-warmed plates | ||
*Transfer cells + DNA onto agar | *Transfer cells + DNA onto agar | ||
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*Incubate plates at 37 °C overnight | *Incubate plates at 37 °C overnight | ||
[[Image:plates3_27_2013.jpg|200px|thumb|left|Ligation attempt 3/27/2013]] | |||
*''No colonies on any of the plates. Try again.'' | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 18:21, 6 September 2013
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3/27/2013
Transformation Process
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