Haynes Lab:Notebook/Engineering PC-TFs/2013/03/27: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
(8 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: # | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: # | |style="background-color: #800000" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 34: | Line 34: | ||
|10.84 | |10.84 | ||
|- | |- | ||
|}<br><br><br><br><br><br><br><br> | |}<br><br><br><br><br><br><br><br> | ||
{| {{table}} | |||
|align="center" style="background:#f0f0f1;"|'''µL of insert = (length insert/lengthvector *2*25 ng vector/concentration insert)''' | |||
|} | |||
{| {{table}} | {| {{table}} | ||
Line 69: | Line 73: | ||
*Add 30 μL of DH5α Turbo cells to DNA + dH2O | *Add 30 μL of DH5α Turbo cells to DNA + dH2O | ||
*Include #5 tube, water only, no DNA (negative control) | *Include #5 tube, water only, no DNA (negative control) | ||
*Incubate cells + DNA on ice for | *Incubate cells + DNA on ice for 10 min. | ||
*Label pre-warmed plates | *Label pre-warmed plates | ||
*Transfer cells + DNA onto agar | *Transfer cells + DNA onto agar | ||
Line 75: | Line 79: | ||
*Incubate plates at 37 °C overnight | *Incubate plates at 37 °C overnight | ||
[[Image:plates3_27_2013.jpg|200px|Ligation attempt 3/27/2013]] | [[Image:plates3_27_2013.jpg|200px|thumb|left|Ligation attempt 3/27/2013]] | ||
*''No colonies on any of the plates. Try again.'' | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 18:21, 6 September 2013
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3/27/2013
Transformation Process
|