Haynes Lab:Notebook/Engineering PC-TFs/2013/03/27: Difference between revisions
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* Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes. | * Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes. | ||
* Then follow transformation process: | * Then follow transformation process: | ||
'''Transformation Process''' | |||
*Warm five 100 μg/mL Amp agar plates at 37 °C | |||
*Thaw fresh tube of DH5α Turbo cells on ice | |||
*Add 30 μL of DH5α Turbo cells to DNA + dH2O | |||
*Include #5 tube, water only, no DNA (negative control) | |||
*Incubate cells + DNA on ice for 5 min. | |||
*Label pre-warmed plates | |||
*Transfer cells + DNA onto agar | |||
*Add 10 - 15 sterile glass beads, shake, discard beads | |||
*Incubate plates at 37 °C overnight | |||
Revision as of 09:08, 2 April 2013
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3/27/2013
Transformation Process
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