Haynes Lab:Notebook/Engineering PC-TFs/2013/09/12: Difference between revisions
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| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. | ||
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''Zymoclean<sup>TM</sup> Gel DNA Recovery Kit'' | |||
*Add 3 volumes of ADB Buffer to each volume of gel (600 μL). | |||
*Incubate at 55°C for 10 minutes. | |||
*Centrifuge at ≥ 10,000 x g for 30 seconds. Discard flow-through. | |||
*Add 200 μL of DNA Wash Buffer to the column and centrifuge for 30 seconds. Repeat wash step. | |||
*Place Zymoclean Column into a new 1.5 mL tube. Add 20μL of dH<sub>2</sub>O to elute DNA | |||
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Revision as of 21:19, 11 October 2013
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September 12, 2013
ZymocleanTM Gel DNA Recovery Kit
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