Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17: Difference between revisions
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==September 17, 2013 == | ==September 17, 2013 == | ||
* | *Restriction Digest of CMV promoter | ||
**for PEG DNA precipitation | |||
{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | {| {{table}} cellspacing="3" <!-- Digest rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | '''Reagent''' | | bgcolor=#cfcfcf | '''Reagent''' | ||
| bgcolor=#cfcfcf | '''Volume''' | | bgcolor=#cfcfcf | '''Volume''' | ||
|- | |- | ||
| DNA (plasmid) || | | DNA (plasmid) || 10 μL | ||
|- | |- | ||
| | | XbaI|| 1.0 μL | ||
|- | |- | ||
| PstI|| 1.0 μL | | PstI|| 1.0 μL | ||
| Line 22: | Line 22: | ||
| Green 10x FastDigest buffer || 3.0 μL | | Green 10x FastDigest buffer || 3.0 μL | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 15 μL | ||
|- | |- | ||
| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. | ||
Revision as of 20:48, 17 September 2013
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