Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17

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(September 17, 2013)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==September 17, 2013 ==
==September 17, 2013 ==
-
*  
+
*Restriction Digest of CMV promoter
 +
**for PEG DNA precipitation
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
|- valign="top"
|- valign="top"
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Volume'''
| bgcolor=#cfcfcf | '''Volume'''
-
 
|-
|-
-
| DNA (plasmid) || 20.0 μL
+
| DNA (plasmid) || 10 μL
|-
|-
-
| SpeI|| 1.0 μL
+
| XbaI|| 1.0 μL
|-
|-
| PstI|| 1.0 μL
| PstI|| 1.0 μL
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| Green 10x FastDigest buffer || 3.0 μL
| Green 10x FastDigest buffer || 3.0 μL
|-
|-
-
| dH<sub>2</sub>O || 5 μL
+
| dH<sub>2</sub>O || 15 μL
|-
|-
| &nbsp; || 30 μL --> 37°C/ ~10 min.
| &nbsp; || 30 μL --> 37°C/ ~10 min.

Revision as of 22:48, 17 September 2013



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September 17, 2013

  • Restriction Digest of CMV promoter
    • for PEG DNA precipitation
Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.


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