Haynes Lab:Notebook/Engineering PC-TFs/2013/09/21: Difference between revisions
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== | ==September 21, 2013== | ||
* | * Although there was no pellet, ran a DNA Clean & Concentrator on samples. | ||
**Add 2 volumes of DNA Binder Buffer to each DNA sample (200 μL). | |||
**Place mixture in spin column into 2 ml collection tube. | |||
**Centrifuge at full speed for 30 seconds. | |||
**Add 20 μL of DNA Wash Buffer and centrifuge for 30 seconds. Repeat this step. | |||
**Place Zymo-Spin Column into a new 1.5 ml tube. Add 20 μL of water directly to column matrix and spin to elute DNA. | |||
*Ran samples on agarose gel. | |||
** Gel is a day old, probably not an accurate testing to see if PEG/MgCl<sub>2</sub> method works. | |||
[[Image:noresults.jpg|250px|PEG MgCl<sub>2</sub>9/21/2013]] | |||
Revision as of 18:29, 26 September 2013
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September 21, 2013
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