Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26: Difference between revisions
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== | ==September 26, 2013== | ||
''Restriction Digest'' | |||
*Perform restriction digest for CMV promoter | *Perform restriction digest for CMV promoter | ||
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*Centrifuge for 15 minutes at 10,000g. | *Centrifuge for 15 minutes at 10,000g. | ||
''DNA Clean & Concentrator'' | |||
* Although there was no pellet, ran a DNA Clean & Concentrator on samples. | |||
**Add 2 volumes of DNA Binder Buffer to each DNA sample (200 μL). | |||
**Place mixture in spin column into 2 ml collection tube. | |||
**Centrifuge at full speed for 30 seconds. | |||
**Add 20 μL of DNA Wash Buffer and centrifuge for 30 seconds. Repeat this step. | |||
**Place Zymo-Spin Column into a new 1.5 ml tube. Add 20 μL of water directly to column matrix and spin to elute DNA. | |||
''Gel Electrophoresis'' | |||
* Follow steps for gel electrophoresis. | * Follow steps for gel electrophoresis. | ||
* Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. | * Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. |
Revision as of 19:32, 26 September 2013
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September 26, 2013Restriction Digest
DNA Clean & Concentrator
Gel Electrophoresis
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