Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26: Difference between revisions
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Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC342844/ Lis and Schleif 1975, NAR]. I kept DNA from the supernatant and discarded the precipitated DNA. | Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC342844/ Lis and Schleif 1975, NAR]. I kept DNA from the supernatant and discarded the precipitated DNA. | ||
Therefore, I should see '''larger''' bands at 2% PEG and '''smaller''' bands at 15% PEG. | Therefore, I should see '''larger?''' bands at 2% PEG and '''smaller?''' bands at 15% PEG. | ||
Revision as of 12:20, 28 September 2013
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September 26, 2013Restriction Digest
PEG/MgCl2 Precipitation
DNA Clean & Concentrator
Gel Electrophoresis
Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to Lis and Schleif 1975, NAR. I kept DNA from the supernatant and discarded the precipitated DNA. Therefore, I should see larger? bands at 2% PEG and smaller? bands at 15% PEG.
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