Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26

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  • Perform restriction digest for CMV promoter
Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.

  • Measure the following into 1.5 mL tubes.
Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL15 μL15 μL15 μL15 μL
dH2O 35 μL52 μL62 μL68 μL78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL
  • Centrifuge for 15 minutes at 10,000g.

  • Follow steps for gel electrophoresis.
  • Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray.
  • Fill gel flask with up to 60 ml of TA buffer.
  • Create 1% gel by putting .6 grams of agarose into flask.
  • Microwave agarose solution for 40 seconds
  • Using hot gloves, grab flask out of microwave and swirl flask vigorously. Place back in microwave for 40 more seconds.
  • When flask is taken out of microwave, make sure that the agarose is completed dissolved.
  • Set up wells on gel electrophoresis apparatus. The mold for the first digest is B-10. Use the thick "teeth" and slip in the notches.
  • Add EtBr (ethidium bromide) to the agarose gel. There should be 1 microliter of EtBr per 10ml of agarose gel. Therefore, 6 µL.
  • Pour gel into tray.
  • Wash the agarose gel flask.
  • Run gel for 45 min at 100 V. Check gel under UV light.

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