Haynes Lab:Notebook/Engineering PC-TFs/2013/10/05: Difference between revisions
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* PEG/MgCl<sub>2</sub> | * PEG/MgCl<sub>2</sub> | ||
** Make 50 mL | ** Make 50 mL | ||
***Put water, then | ***Put water, then MgCl<sub>2</sub>, then PEG 8000 in 50 mL conical tube | ||
{|border="1" cellpadding="6" cellspacing="0" align="left" | {|border="1" cellpadding="6" cellspacing="0" align="left" |
Revision as of 21:06, 11 October 2013
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October 5, 2013
PEG/MgCl2 Precipitation
DNA Clean & Concentrator
Gel Electrophoresis
Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to Lis and Schleif 1975, NAR. I kept DNA from the supernatant and discarded the precipitated DNA. Therefore, I should see larger bands at 2% PEG and smaller bands at 15% PEG.
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