Haynes Lab:Notebook/Engineering PC-TFs/2013/10/11: Difference between revisions
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==October 11, 2013== | ==October 11, 2013== | ||
'''Restriction Digest''' | |||
''Gel Electrophoresis'' | |||
* Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. | * Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. | ||
* Fill gel flask with up to 60 ml of TA buffer. | * Fill gel flask with up to 60 ml of TA buffer. | ||
Line 38: | Line 41: | ||
| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. | ||
|} | |} | ||
''Zymoclean<sup>TM</sup> Gel DNA Recovery Kit'' | |||
*Add 3 volumes of ADB Buffer to each volume of gel (600 μL). | |||
*Incubate at 55°C for 10 minutes. | |||
*Centrifuge at ≥ 10,000 x g for 30 seconds. Discard flow-through. | |||
*Add 200 μL of DNA Wash Buffer to the column and centrifuge for 30 seconds. Repeat wash step. | |||
*Place Zymoclean Column into a new 1.5 mL tube. Add 20μL of dH<sub>2</sub>O to elute DNA | |||
{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
|- | |- | ||
! scope="col" style="background:#efefef;" | # | ! scope="col" style="background:#efefef;" | # | ||
! scope="col" style="background:#efefef;" | | ! scope="col" style="background:#efefef;" | Part | ||
! scope="col" style="background:#efefef;" | ng/µL | ! scope="col" style="background:#efefef;" | ng/µL | ||
|- | |- | ||
|1 | |1 | ||
|CMV | |CMV | ||
| | |0.851 | ||
|- | |- | ||
|2 | |2 | ||
|CMV | |CMV | ||
| | |15.535 | ||
|} | |} | ||
Revision as of 21:18, 11 October 2013
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October 11, 2013Restriction Digest Gel Electrophoresis
ZymocleanTM Gel DNA Recovery Kit
|