Haynes Lab:Notebook/Engineering PC-TFs/2013/10/11: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav) |
|||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==October 11, 2013== | ==October 11, 2013== | ||
'''Restriction Digest''' | |||
''Gel Electrophoresis'' | |||
* Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. | * Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. | ||
* Fill gel flask with up to 60 ml of TA buffer. | * Fill gel flask with up to 60 ml of TA buffer. | ||
Line 38: | Line 41: | ||
| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. | ||
|} | |} | ||
''Zymoclean<sup>TM</sup> Gel DNA Recovery Kit'' | |||
*Add 3 volumes of ADB Buffer to each volume of gel (600 μL). | |||
*Incubate at 55°C for 10 minutes. | |||
*Centrifuge at ≥ 10,000 x g for 30 seconds. Discard flow-through. | |||
*Add 200 μL of DNA Wash Buffer to the column and centrifuge for 30 seconds. Repeat wash step. | |||
*Place Zymoclean Column into a new 1.5 mL tube. Add 20μL of dH<sub>2</sub>O to elute DNA | |||
{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
|- | |- | ||
! scope="col" style="background:#efefef;" | # | ! scope="col" style="background:#efefef;" | # | ||
! scope="col" style="background:#efefef;" | | ! scope="col" style="background:#efefef;" | Part | ||
! scope="col" style="background:#efefef;" | ng/µL | ! scope="col" style="background:#efefef;" | ng/µL | ||
|- | |- | ||
|1 | |1 | ||
|CMV | |CMV | ||
| | |0.851 | ||
|- | |- | ||
|2 | |2 | ||
|CMV | |CMV | ||
| | |15.535 | ||
|} | |} | ||
Latest revision as of 23:26, 26 September 2017
Main project page Previous entry Next entry | |||||||||||||||||||||||||
October 11, 2013Restriction Digest Gel Electrophoresis
ZymocleanTM Gel DNA Recovery Kit
|