Haynes Lab:Notebook/Engineering PC-TFs/2013/10/19: Difference between revisions
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==October 19, 2013== | ==October 19, 2013== | ||
''Ligation of CMV + MV9'' | |||
{| {{table}} | |||
| align="center" style="background:#efefef;"| | |||
| align="center" style="background:#efefef;"|'''1''' | |||
| align="center" style="background:#efefef;"|'''Control''' | |||
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| DNA Insert||1.4||1.8 | |||
|- | |||
| DNA Vector (Kozak)||0.6||0.6 | |||
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| T4 Ligase||1||1 | |||
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| Lign Buffer (2x)||5||5 | |||
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| dH2O||2.0||1.6 | |||
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| --||10 µL||10 µL | |||
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''Bacterial Transformation of CMV Promoter + MV9'' | ''Bacterial Transformation of CMV Promoter + MV9'' | ||
*Warm 100 μg/mL Amp agar plate at 37°C | *Warm 100 μg/mL Amp agar plate at 37°C |
Revision as of 14:07, 20 October 2013
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October 19, 2013Ligation of CMV + MV9
Incubate plates at 37°C overnight and retrieve next day.
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