Haynes Lab:Notebook/Engineering PC-TFs/2013/10/19: Difference between revisions
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| align="center" style="background:#efefef;"|'''Control''' | | align="center" style="background:#efefef;"|'''Control''' | ||
|- | |- | ||
| DNA Insert|| | | DNA Insert||0.8||0 | ||
|- | |- | ||
| DNA Vector ( | | DNA Vector (MV9)||2.4||2.4 | ||
|- | |- | ||
| T4 Ligase||1||1 | | T4 Ligase||1||1 | ||
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| Lign Buffer (2x)||5||5 | | Lign Buffer (2x)||5||5 | ||
|- | |- | ||
| | | dH<sub>2</sub>O ||0.8||1.6 | ||
|- | |- | ||
| '''Total'''||10 µL||10 µL | | '''Total'''||10 µL||10 µL |
Revision as of 14:08, 20 October 2013
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October 19, 2013Ligation of CMV + MV9
Incubate plates at 37°C overnight and retrieve next day.
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