Haynes Lab:Notebook/Engineering PC-TFs/2013/10/19: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 32: | Line 32: | ||
*Warm 100 μg/mL Amp agar plate at 37°C | *Warm 100 μg/mL Amp agar plate at 37°C | ||
*Thaw fresh tube of DH5α Turbo cells on ice | *Thaw fresh tube of DH5α Turbo cells on ice | ||
*Add 30 μL of DH5α Turbo Cells to DNA + dH<sub>2</sub>O | *Add 30 μL of DH5α Turbo Cells to DNA + dH<sub>2</sub>O. | ||
** This was also done for transformation of part KAH126 | |||
*Incubate cells + DNA on ice for 5 minutes. | *Incubate cells + DNA on ice for 5 minutes. | ||
*Label pre-warmed plate. | *Label pre-warmed plate. | ||
Line 38: | Line 39: | ||
*Add 8-10 glass beads, shake, then discard beads. | *Add 8-10 glass beads, shake, then discard beads. | ||
Incubate plates at 37°C overnight and retrieve next day. | Incubate plates at 37°C overnight and retrieve next day. | ||
Revision as of 14:09, 20 October 2013
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||
October 19, 2013Ligation of CMV + MV9
Incubate plates at 37°C overnight and retrieve next day.
|