Haynes Lab:Notebook/Engineering PC-TFs/2013/10/19: Difference between revisions
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| CMV||15.535 ng/μL | | CMV (insert)||15.535 ng/μL | ||
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| MV9||20.838 ng/μL | | MV9 (vector)||20.838 ng/μL | ||
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|} | |} | ||
<span style="font-size:95%"> | <span style="font-size:95%">''Calculations'' | ||
ng insert = bp insert/bp vector * 2 * 50 | |||
ng insert = 588/4611 * 2 *50 = 12.75211 ng | |||
μL insert = ng insert/concentration insert | |||
μL insert = 12.75211/15.535 = .82μL | |||
μL vector = 50 ng/concentration vector | |||
μL vector = 50/20.838 = 2.4 μL<span style="font-size:95%"> | |||
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Revision as of 14:16, 20 October 2013
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October 19, 2013Ligation of CMV + MV9
Calculations ng insert = bp insert/bp vector * 2 * 50 ng insert = 588/4611 * 2 *50 = 12.75211 ng μL insert = ng insert/concentration insert μL insert = 12.75211/15.535 = .82μL μL vector = 50 ng/concentration vector μL vector = 50/20.838 = 2.4 μL
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