Haynes Lab:Notebook/Engineering PC-TFs/2013/10/20

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==October 20,2013==
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==October 20, 2013==
''Grow liquid culture of CMV + MV9, KAH126''
''Grow liquid culture of CMV + MV9, KAH126''
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
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[[Image:supercali.jpg|200px|Bacterial Transformation of KAH126 and Ligation of CMV + MV9]]
[[Image:supercali.jpg|200px|Bacterial Transformation of KAH126 and Ligation of CMV + MV9]]
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*<span style="font-size:95%"> Bacterial transformation of KAH126 was successful. Restriction digest will need to be to confirm successful ligation. Additionally, presence of film showed on control. Reason for contamination is unknown.

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October 20, 2013

Grow liquid culture of CMV + MV9, KAH126

  • Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
  • Use sterile pipette tip to touch bacterial streak and put the tip into LB medium.
  • Grow the cultures for 10-12 hrs.

Bacterial Transformation of KAH126 and Ligation of CMV + MV9

  • Bacterial transformation of KAH126 was successful. Restriction digest will need to be to confirm successful ligation. Additionally, presence of film showed on control. Reason for contamination is unknown.


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