Haynes Lab:Notebook/Engineering PC-TFs/2013/10/20: Difference between revisions

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==October 20,2013==
==October 20, 2013==
''Grow liquid culture of CMV + MV9, KAH126''
''Grow liquid culture of CMV + MV9, KAH126''
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
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[[Image:supercali.jpg|200px|Bacterial Transformation of KAH126 and Ligation of CMV + MV9]]
[[Image:supercali.jpg|200px|Bacterial Transformation of KAH126 and Ligation of CMV + MV9]]


*<span style="font-size:95%"> Bacterial transformation of KAH126 was successful. Restriction digest will need to be to confirm successful ligation. Additionally, presence of film showed on control. Reason for contamination is unknown.




Latest revision as of 23:29, 26 September 2017



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October 20, 2013

Grow liquid culture of CMV + MV9, KAH126

  • Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
  • Use sterile pipette tip to touch bacterial streak and put the tip into LB medium.
  • Grow the cultures for 10-12 hrs.

Bacterial Transformation of KAH126 and Ligation of CMV + MV9

  • Bacterial transformation of KAH126 was successful. Restriction digest will need to be to confirm successful ligation. Additionally, presence of film showed on control. Reason for contamination is unknown.



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