Haynes Lab:Notebook/Engineering PC-TFs/2013/10/21: Difference between revisions
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==October 21, 2013== | ==October 21, 2013== | ||
<big>''Miniprep liquid cultures''</big> | |||
*Add 100 μL of 7X Lysis Buffer (Blue) to 600 μL of E.coli culture in a 1.5 ml microcentrifuge tube. Mix by inverting tube 4-6times. | *Add 100 μL of 7X Lysis Buffer (Blue) to 600 μL of E.coli culture in a 1.5 ml microcentrifuge tube. Mix by inverting tube 4-6times. | ||
*Add 350 μL of cold Neutralization Buffer (Yellow), mix thoroughly. Neutralization is complete when sample becomes yellow and precipitate has formed. | *Add 350 μL of cold Neutralization Buffer (Yellow), mix thoroughly. Neutralization is complete when sample becomes yellow and precipitate has formed. |
Revision as of 14:13, 25 October 2013
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October 21, 2013Miniprep liquid cultures
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