Haynes Lab:Notebook/Engineering PC-TFs/2014/04/08: Difference between revisions
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(Autocreate 2014/04/08 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs) |
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==Summary== | ==Summary== | ||
* | * PCR Reaction | ||
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| bgcolor=#cfcfcf | Reagent | |||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1. PCR Reaction Product = 845bp <br> | |||
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| DNA(CMV/MV9) || 0.5 μL | |||
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| 2X GoTaq || 12.5 μL | |||
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| FP DD122 B || 1.0 μL | |||
|- | |||
| RP DD123 B || 1.0 μL | |||
|- | |||
| dH<sub>2</sub>O || 10.0 μL | |||
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*Placed reaction mixture into PCR machine on setting GOTAQ35cyc | |||
*Gel verification | |||
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| rowspan="7" | [[Image:2014-04-10 15.47.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; | |||
[http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
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*Expected base pair length: 643 | |||
<br> <br> | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 18:13, 18 April 2014
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Summary
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