Haynes Lab:Notebook/Engineering PC-TFs/2014/04/18: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 50: | Line 50: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. CMV = | | rowspan="7" | <u>Expected:</u><br>1. CMV = 643bp <br> | ||
|- | |- | ||
| DNA(CMV/MV9) || 4.0 μL | | DNA(CMV/MV9) || 4.0 μL | ||
Line 69: | Line 69: | ||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| rowspan="7" | [[Image:2014-04-18 13.13.10.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; | | rowspan="7" | [[Image:2014-04-18 13.13.10.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; | ||
[http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|} | |} | ||
*Expected base pair length: 643 | *Expected base pair length: 643 | ||
__NOTOC__ | __NOTOC__ |
Revision as of 16:48, 18 April 2014
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||
Summary4/16
4/17
4/18
|
- Restriction Digest
Reagent | Volume | Expected: 1. CMV = 643bp |
DNA(CMV/MV9) | 4.0 μL | |
10X buffer | 1.5 μL | |
NotI | 1.0 μL | |
NruI | 1.0 μL | |
dH2O | 7.5 μL | |
15 μL --> 37°C/ 15 min. |
- Verified for the sake of not wasting money on sequencing.
15 μL/lane; 1% agarose; Ladder |
- Expected base pair length: 643