Haynes Lab:Notebook/Engineering PC-TFs/2014/04/18: Difference between revisions
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*Expected base pair length: 643 | *Expected base pair length: 643 | ||
*Observed base pair length: ~650 | |||
<br> <br> | <br> <br> | ||
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| Plasmid Title || Primer || Water || Plasmid (~200ng/uL) | | Plasmid Title || Primer || Water || Plasmid (~200ng/uL) | ||
|- | |- | ||
| 1. cmvmv9f1 || 1uL DD122 B || 8uL || 1uL | | 1. cmvmv9f1 || FP 1uL DD122 B || 8uL || 1uL | ||
|- | |- | ||
| 2. cmvmv9r1 || 1uL DD123 B || 8uL || 1uL | | 2. cmvmv9r1 || RP 1uL DD123 B || 8uL || 1uL | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Revision as of 19:35, 18 April 2014
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Summary4/16
4/17
4/18
|
- Restriction Digest
Reagent | Volume | Expected: 1. CMV = 643bp |
DNA(CMV/MV9) | 4.0 μL | |
10X buffer | 1.5 μL | |
NotI | 1.0 μL | |
NruI | 1.0 μL | |
dH2O | 7.5 μL | |
15 μL --> 37°C/ 15 min. |
- Verified for the sake of not wasting money on sequencing.
15 μL/lane; 1% agarose; Ladder |
- Expected base pair length: 643
- Observed base pair length: ~650
- Sent off for sequencing
Plasmid Title | Primer | Water | Plasmid (~200ng/uL) |
1. cmvmv9f1 | FP 1uL DD122 B | 8uL | 1uL |
2. cmvmv9r1 | RP 1uL DD123 B | 8uL | 1uL |