Haynes Lab:Notebook/Engineering PC-TFs/2014/04/18
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Summary4/16
4/17
4/18
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- Restriction Digest
Reagent | Volume | Expected: 1. CMV = 643bp |
DNA(CMV/MV9) | 4.0 μL | |
10X buffer | 1.5 μL | |
NotI | 1.0 μL | |
NruI | 1.0 μL | |
dH2O | 7.5 μL | |
15 μL --> 37°C/ 15 min. |
- Verified for the sake of not wasting money on sequencing.
15 μL/lane; 1% agarose; Ladder |
- Expected base pair length: 643
- Sent off for sequencing
Plasmid Title | Primer | Water | Plasmid (~200ng/uL) |
1. cmvmv9f1 | 1uL DD122 B | 8uL | 1uL |
2. cmvmv9r1 | 1uL DD123 B | 8uL | 1uL |