Haynes Lab:Notebook/Engineering PC-TFs/2014/06/23

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'''CMV/MV9, BL05, BL09 retransformation'''
'''CMV/MV9, BL05, BL09 retransformation'''
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I am starting over with my CMV/MV9 ligation so I decided to retransform all components though only BL05 had a low concentration in previous miniprep. Followed standard transformation procedure in cloning bootcamp with the exception of a longer incubation time and the use of 60μL cells rather than 50μL.
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I am starting over with my CMV/MV9 PcTF ligation so I decided to retransform all components though only BL05 had a low concentration in previous miniprep. Followed standard transformation procedure in cloning bootcamp with the exception of a longer incubation time and the use of 60μL cells rather than 50μL.
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Summary

CMV/MV9, BL05, BL09 retransformation

I am starting over with my CMV/MV9 PcTF ligation so I decided to retransform all components though only BL05 had a low concentration in previous miniprep. Followed standard transformation procedure in cloning bootcamp with the exception of a longer incubation time and the use of 60μL cells rather than 50μL.


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