Haynes Lab:Notebook/Engineering PC-TFs/2014/06/23: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/06/23 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Summary==
==Summary==
*
'''CMV/MV9, BL05, BL09 retransformation'''
 
<br> <br>
I am starting over with my CMV/MV9 ligation so I decided to retransform all components though only BL05 had a low concentration in previous miniprep. Followed standard transformation procedure in cloning bootcamp with the exception of a longer incubation time and the use of 60μL cells rather than 50μL.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 16:33, 25 June 2014



<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

CMV/MV9, BL05, BL09 retransformation

I am starting over with my CMV/MV9 ligation so I decided to retransform all components though only BL05 had a low concentration in previous miniprep. Followed standard transformation procedure in cloning bootcamp with the exception of a longer incubation time and the use of 60μL cells rather than 50μL.