Summary
Liquid Culture Minipreps
Eluted with 70μL dH2O.
Plasmid |
OD260 |
OD260/280 |
ng/μL
|
1. BL01 C1 |
0.289 |
1.89 |
288.704
|
1. BL01 C2 |
0.463 |
1.918 |
462.857
|
1. BL01 C3 |
0.495 |
1.915 |
495.398
|
Plasmid |
OD260 |
OD260/280 |
ng/μL
|
1. BL05 C1 |
0.047 |
2.124 |
46.727
|
1. BL05 C2 |
0.301 |
1.912 |
301.14
|
1. BL05 C3 |
0.075 |
1.97 |
74.969
|
Plasmid |
OD260 |
OD260/280 |
ng/μL
|
1. BL09 C1 |
0.059 |
1.986 |
59.185
|
1. BL09 C2 |
0.236 |
1.878 |
235.782
|
1. BL09 C3 |
0.185 |
1.958 |
184.993
|
Restriction Digest
Reagent
|
Volume
|
Expected: BL01(W1:2-4), BL05(W1:5, W2:2-3) Forward insertion: 710, 7010. Reverse insertion: 3230, 4490. No insertion: 710, 4490. BL09 (W2:4-6) Forward insertion: 710, 5921 Reverse insertion: 2141, 4490 No insertion: 710, 4490. *Final well is empty CMV/MV9.
|
15 μL/lane; 1% agarose; Observed lengths: W2: 7100, 700; W3: 7100, 5200, 2500; W4: *5200, 2500, 710; W5: 5000, 2500; W6: 5000, 2500, 710. *Uncut plasmid. Ladder
|
15 μL/lane; 1% agarose; Observed lengths: W2: *7100, 700; W3: *6100, 2500, 710; W4: *7200, 2500, 710; W5: 5000, 4000; W6: *5500, 2500, 750. *Uncut plasmid. Ladder
|
DNA(BL01x3, BL05x3, BL09x3, CMV/MV9) |
5.0 μL
|
10X buffer |
1.5 μL
|
XbaI |
1.0 μL
|
NruI |
1.0 μL
|
dH2O |
6.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Results
Top W2: BL01 possible success.
Bottom W2: BL05 possible success.
Bottom W4: BL09 possible success.
Sequencing Results Order 9218
BL01rA
- Used 1μL BL01 miniprep 1, 1μL DD123 B primer, 8μLdH2O.
- Results: Matches were found after the primer and then just before CMV suggesting no insert.
BL01rB
- Used 1μL BL01 miniprep 2, 1μL DD123 B primer, 8μLdH2O.
- Results: Matches were found after the primer and then just before CMV suggesting no insert.
BL09rA
- Used 1μL BL09 miniprep 1, 1μL DD123 B primer, 8μLdH2O.
- Results: Matches were found after the primer and then just before CMV suggesting no insert.
|