Haynes Lab:Notebook/Engineering PC-TFs/2014/08/01: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav) |
|||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 14: | Line 14: | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="9" | | | rowspan="9" | | ||
<u>Expected:</u> | <u><b>Expected:</b></u> | ||
<br> | <br> | ||
BL09 Ligation Colonies 1-3[XbaI/NruI] (Well: 2-4)<br> | <u>BL09 Ligation Colonies 1-3[XbaI/NruI] (Well: 2-4)</u><br> | ||
Forward insertion: 710, 5921 <br> | Forward insertion: 710, 5921 <br> | ||
Reverse insertion: 2141, 4490 <br> | Reverse insertion: 2141, 4490 <br> | ||
No insertion: 710, 4490<br> <br> | No insertion: 710, 4490<br> <br> | ||
CMV/MV9 with [XbaI/NruI] (Well: 5) <br> | <u>CMV/MV9 with [XbaI/NruI] (Well: 5)</u> <br> | ||
710, 4490<br><br> | 710, 4490<br><br> | ||
CMV/MV9 [XbaI](Well: 6) <br> | <u>CMV/MV9 [XbaI](Well: 6)</u> <br> | ||
5200<br><br> | 5200<br><br> | ||
Dephos CMV/MV9 (Well: 7) <br> | <u>Dephos CMV/MV9 (Well: 7)</u> <br> | ||
5200 | 5200 | ||
| rowspan="10" | [[Image:20140801 132828.jpg|400px|Today's gel]]<br> | | rowspan="10" | [[Image:20140801 132828.jpg|400px|Today's gel]]<br> | ||
Line 37: | Line 37: | ||
[http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA( | | DNA(BL09x3, CMV/MV9x3) || 3.0 μL | ||
|- | |- | ||
| 10X buffer || 1.5 μL | | 10X buffer || 1.5 μL | ||
|- | |- | ||
| XbaI || 1.0 μL | | XbaI || 1.0 μL(or 0 for dephos CMV/MV9) | ||
|- | |- | ||
| NruI || 1.0 μL | | NruI || 1.0 μL (or 0 CMV/MV9 and dephos CMV/MV9) | ||
|- | |- | ||
| dH<sub>2</sub>O || 8.5 μL | | dH<sub>2</sub>O || 8.5 μL |
Latest revision as of 00:09, 27 September 2017
Main project page Previous entry Next entry | |||||||||||||||||
SummaryLigation Digest and Gel Verification
|