Haynes Lab:Notebook/Engineering PC-TFs/2014/09/15: Difference between revisions
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|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* Centrifuged samples at max rpm for five minutes in cold room. | * Centrifuged samples at max rpm for five minutes in cold room. | ||
* Labeled three new 1.5mL tubes. | * Labeled three new 1.5mL tubes. | ||
* Transferred | * Transferred supernatant to new tube being careful not to disturb the pellet at the bottom of each tube. | ||
* Used in Bradford Assay and stored in the -20°C. | |||
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Bradford assay | Bradford assay | ||
* Followed Bradford Assay protocol: http://openwetware.org/wiki/Haynes:Bradford | |||
* Used 5 μL for each BL09 sample | * Used 5 μL for each BL09 sample | ||
* OD 590 readings: | * OD 590 readings: |
Latest revision as of 00:17, 27 September 2017
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Summary
Protein extraction from transfected cells
Plate reader measurements RFP signal
Standard curve
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