Haynes Lab:Notebook/Engineering PC-TFs/2014/09/15: Difference between revisions
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(Autocreate 2014/09/15 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs) |
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==Summary== | ==Summary== | ||
* | * Protein extraction | ||
* Plate reader measurements: RFP and Bradford (protein) | |||
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'''Protein extraction from transfected cells''' | |||
* Cameron, please add notes here | |||
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'''Plate reader measurements''' | |||
RFP signal | |||
* Followed the fluorescent protein assay | |||
* Results: | |||
# BL09 1 = 109 | |||
# BL09 2 = 610 | |||
# BL09 negative ctrl (no DNA) = 47 | |||
Bradford assay | |||
* Used 5 μL of each sample | |||
# standard 1 (1 ug) = 0.25 0.266 0.295 0.356 0.432 0.608 0.486 0.481 0.471 | |||
Revision as of 14:01, 15 September 2014
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Summary
Protein extraction from transfected cells
Plate reader measurements RFP signal
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